bacterial contamination of donor corneas and corneal storage media received from the eye bank of i.r. iran

purpose: to determine bacterial contamination of corneas and corneal storage media provided from the eye bank of i.r. iran to rasool akram medical center. methods: this descriptive study was performed on 70 corneoscleral rims and corneal storage media. one milliliter of corneal storage media and the corneoscleral rim were sent to microbiology laboratory at the time of penetrating keratoplasty and specimens were cultured on blood agar, emb agar, and thioglycolate broth. results: no organism growth was observed after 72 hours in any of 70 storage media or corneoscleral rims. conclusion: the aseptic protocol of the eye bank of i.r. iran is proper in term of tissue preparation, preservation, and transferring of corneal graft is proper.